ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

SURVEY: Cognitive Impairment II

Top 3 Nutrients to Detox the Liver and Soothe Digestion

Natural Bladder Control, Go Less and Live More

Study bodes well for low-carb eaters

Top Vitamin and Mineral Deficiencies — Are You at Risk?

Omega Fix for Obesity: How the Right Fats Fight Fat

Potential of Quercetin in the Treatment of Melanoma

How Pomegranate May Protect Against Cancer

Vital Molecule Increases Cellular Energy and Improves Cognitive Function

Trimming the spare tire: Canola oil may cut belly fat

 
Print Page
Email Article

Powerful New Method Helps Reveal Genetic Basis of Cancer

  [ 15 votes ]   [ Discuss This Article ]
www.ProHealth.com • September 15, 2003


Plus surprising differences in 'Normal' DNA

Researchers at Cold Spring Harbor Laboratory have developed one of the most sensitive, comprehensive, and robust methods that now exists for profiling the genetic basis of cancer and other diseases. The method detects chromosomal deletions and amplifications (i.e. missing or excess copies of DNA segments), and is useful for a wide variety of biomedical and other applications.

The study, led by Rob Lucito and Michael Wigler, is published in the October issue of the journal Genome Research (advance on-line publication date: September 15). The powerful new gene discovery method described in the study is called ROMA (Representational Oligonucleotide Microarray Analysis).

By using ROMA to compare the DNA of normal cells and breast cancer cells, the researchers have uncovered a striking collection of chromosomal amplifications and deletions that are likely to be involved in some aspect of breast cancer (see http://roma.cshl.org, password available on request). Some of the DNA amplifications and deletions detected in this study correspond to known oncogenes and tumor suppressor genes. However, many of them are likely to reveal new genes and cellular functions involved in breast cancer or cancer in general.

It would be enough that ROMA is capable of providing a comprehensive, genome-wide view of the genetic alterations associated with breast and other cancers. Such information is invaluable for improving the diagnosis and treatment of the disease. But when comparing "normal to normal" instead of "normal to tumor" DNA samples, Lucito and Wigler got a big surprise.

They frequently detected large (100 kb to 1 Mb) chromosomal deletions and duplications in such normal DNA samples. The scientists speculate that such variations or "copy number polymorphisms" among individuals might well underlie many human traits, including heritable predisposition or resistance to disease.

Background Information Relevant to the Development of ROMA

In 1981, using a gene transfer method he pioneered, Wigler, working at Cold Spring Harbor Laboratory, discovered one of the first human oncogenes (the H-ras oncogene). Hyperactive or excess Ras protein, or alterations in cell functions related to Ras, are implicated in the majority of cancers. Today, cancer therapies that target components of the Ras pathway (e.g. farnesyltransferase inhibitors) are in clinical trials.

In 1993, Wigler and Nikolai Lisitsyn developed a powerful and versatile gene discovery method called Representational Difference Analysis (RDA). RDA enables researchers to "clone the differences" between any two sets of DNA (for example, normal versus tumor DNA isolated from the same patient, or uninfected cells versus cells infected with an unknown virus).

Since 1993, Wigler's lab, alone and in collaboration with groups at Columbia University and Tularik, Inc., has used RDA to identify several previously unrecognized oncogenes and tumor suppressors genes, primarily from sporadic breast cancers (the most common form of the disease). These include PTEN and DBC2 (tumor suppressor genes frequently missing from or inactive in a large proportion of breast and other cancers) in addition to KCNK9 (a gene whose identification revealed a previously unrecognized mechanism for oncogene action as well as an attractive therapeutic target, namely, potassium channels).

The new method, ROMA, is essentially "RDA on a chip." Combining RDA and DNA microarray analysis with the third "pillar" of the method--i.e. knowledge of the complete human genome sequence--gives ROMA unprecedented ability to detect copy number fluctuations on a genome-wide scale.


In addition to Lucito and Wigler, others involved with the study include Larry Norton of Memorial Sloan-Kettering Cancer Center and Scott Powers of Tularik, Inc.



Post a Comment

Featured Products From the ProHealth Store
Vitamin D3 Extreme™ Mitochondria Ignite™ with NT Factor® Ultra ATP+, Double Strength


Article Comments



Be the first to comment on this article!

Post a Comment


 
Natural Pain Relief Supplements

Featured Products

Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid
Optimized Curcumin Longvida® Optimized Curcumin Longvida®
Supports Cognition, Memory & Overall Health

Natural Remedies

Optimize Your Immune System Naturally: Thymic Protein A Optimize Your Immune System Naturally: Thymic Protein A
Restore Youthful Cognition and Well-Being Restore Youthful Cognition and Well-Being
Stop Bacteria With Nature's Antibiotics Stop Bacteria With Nature's Antibiotics
SAD? Coping with Seasonal Affective Disorder SAD? Coping with Seasonal Affective Disorder
When a Good Night's Sleep Is Just a Daydream... When a Good Night's Sleep Is Just a Daydream...

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2016 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map