ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Inflammation Disrupts Memory - What Can You Do to Protect Your Brain?

Artificial sweeteners linked to risk of weight gain, heart disease and other health issues

All About Ginkgo Biloba: Benefits of This Timeless Herbal Supplement

Yarrow Oil: Here's Why It Deserves a Place in Your First-Aid Kit

Vitamin D supplement use associated with lower risk of breast cancer

Carnitine deficiency suggested as contributor to autism

Hop Oil: A Safe Sleep Aide

Lutein — An Important Nutrient for Eye and Brain Health

White Camphor Oil: The Purest Camphor Oil

Taurine: Facts About This Crucial Amino Acid

 
Print Page
Email Article

The prevalence of Xenotropic Murine Leukemia Virus-Related Virus in Healthy Blood Donors in Japan [Apparently unpublished, posted by DiagnoseSupport.com]

  [ 31 votes ]   [ Discuss This Article ]
By Rika A. Furuta, et al. • www.ProHealth.com • March 16, 2010


[Note: according to Whittemore Peterson Institute, this Japanese study “used WPI techniques of PBMC culture and serology for positive detection of XMRV in blood samples.”]

To estimate the impacts of infection with xenotropic murine leukemia virus-related virus (XMRV) on the blood service, we investigated the prevalence of this virus in both prostate cancer patients and healthy blood donors in Japan. All specimens from the prostate cancer patients were collected after obtaining their written informed consent. The ethical committee of the Japanese Red Cross Society approved the examination of XMRV antibodies, but not nucleic acids, in random donor sera.

All serum samples of healthy blood donors tested negative for HIV-1, HIV-2, HTLV-1, hepatitis B virus, hepatitis C virus and human parvovirus B19.

To make a recombinant virus as test antigens for the antibody screening, 293T cells were transfected with an expression vector carrying an XMRV provirus clone, namely, VP62 (kindly gifted by Dr. R.H. Silverman). We used an env-defective mutant of HIV-1 derived from pNL4-3 (kindly gifted by Dr. A. Adachi) as a negative control.

Two days after transfection, the culture supernatants of the transfected cells were collected and concentrated 20 times by centrifugation. We implemented western blotting assay to screen antibodies against XMRV in sera because a high background was observed if we performed enzyme-linked immunosorbent assay. In the western blotting, the blot strips were incubated with the serum samples diluted 1:100 with 5 % skim milk in Tris-buffered saline overnight at
4°C.

Two of 32 serum samples collected from the prostate cancer patients and 5 of 300 serum samples collected from healthy blood donors tested positive for antibodies against XMRV Gag protein. We did not observe any specific signals against Env proteins in the western blotting. Of the 2 serum samples that were obtained from prostate cancer patient and tested positive for anti-XMRV antibodies, the XMRV specific nucleic acid sequence was detected in only one sample (patient #24) by using nested RT-PCR.

In addition, we collected 7 mL of whole blood cells from the patient #24 and cultured the peripheral blood mononuclear cells (PBMCs) in the presence of recombinant interleukin 2 and concanavalin A.

The PBMCs were harvested after 10 days of culture, the virus was isolated from the cells by performing a LacZ marker rescue assay and RNA and genomic DNA were extracted. The nested PCR performed to detect XMRV yielded positive results for both genomic and RT PCR, although the virus was successfully isolated in only 1 of 3 independent experiments. To examine the susceptibility of PBMCs derived from healthy individuals to XMRV, we inoculated activated PBMCs from 3 healthy volunteers with the culture supernatant of the PBMCs obtained from patient #24. By using the nested PCR, we detected the XMRV-specific nucleic acid sequence in the genomic DNA of the PBMCs obtained from 2 of the 3 healthy volunteers.

We conclude that XMRV infection is prevalent among both prostate cancer patients and healthy individuals in Japan. Although our study had a limited sample size, the prevalence among blood donors as determined by identifying XMRV-specific antibodies was found to be 1.7%, while that among prostate cancer patients was found to be 6.3% (P<0.05, one-sided Mann-Whitney U-test).

The results of genomic PCR performing on the PBMCs indicate that XMRV is sustained in a few fractions of blood cells and can spread through blood even though the virus replication rate appears to be very low.

Source: Reported by Whittemore Peterson, posted on DiagnoseSupport.com. Furuta RA, Miyazawa T, Sugiyama T, Kimura T, Hirayama F, Tani Y, Shibata H. Department of Research, Japanese Red Cross Osaka Blood Center; Laboratory for Viral pathogenesis, Institute for Virus Research, Kyoto University; Department of Urology, Nishiwaki Municipal Hospital.





Post a Comment

Featured Products From the ProHealth Store
FibroSleep™ Ultra EPA  - Fish Oil Ultra ATP+, Double Strength


Article Comments



Be the first to comment on this article!

Post a Comment


 
NAD+ Ignite with Niagen

Featured Products

Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Optimized Curcumin Longvida® Optimized Curcumin Longvida®
Supports Cognition, Memory & Overall Health
Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%

Natural Remedies

The Brain Boosting and Fatigue Fighting B-12 The Brain Boosting and Fatigue Fighting B-12
When a Good Night's Sleep Is Just a Daydream... When a Good Night's Sleep Is Just a Daydream...
Undenatured Type II Collagen - Chicken Soup for Your Joints Undenatured Type II Collagen - Chicken Soup for Your Joints
The Remarkable Benefits of Reishi Medicinal Mushrooms The Remarkable Benefits of Reishi Medicinal Mushrooms
Secret Nutrient for Radiant Skin Secret Nutrient for Radiant Skin

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map