J Ethnopharmacol. 2003 Oct;88(2-3):205-14.
Yin W, Wang TS, Yin FZ, Cai BC.
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, 282 Hanzhong Road, 210029, Nanjing, PR China
To further understand the purpose of the traditional processing method of the seeds of Strychnos nux-vomica L. (Loganiaceae) as well as analgesic and anti-inflammatory activities of brucine and brucine N-oxide extracted from this medicinal plant, various pain and inflammatory models were employed in the present study to investigate their pharmacological profiles. Both brucine and brucine N-oxide revealed significant protective effects against thermic and chemical stimuli in hot-plate test and writhing test. However, on different phases they exerted analgesic activities in formalin test.
Brucine N-oxide showed stronger inhibitory effect than brucine in carrageenan-induced rat paw edema, both of them significantly inhibited the release of prostaglandin E(2) in inflammatory tissue, reduced acetic acid-induced vascular permeability and the content of 6-keto-PGF(1a) in Freund’s complete adjuvant (FCA) induced arthritis rat’s blood plasma.
In addition, brucine and brucine N-oxide were shown to reduce the content of 5-hydroxytryptamine (5-HT) in FCA-induced arthritis rat’s blood plasma, while increase the content of 5-hydroxytryindole-3-acetic acid (5-HIAA) accordingly. These results suggest that central and peripheral mechanism are involved in the pain modulation and anti-inflammation effects of brucine and brucine N-oxide, biochemical mechanisms of brucine and brucine N-oxide are different even though they are similar in chemical structure.
PMID: 12963144 [PubMed – in process]