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White-footed mice (Peromyscus leucopus) were captured and their tissues sampled from 27 sites in seven counties of western Pennsylvania in 1990 for isolation and identification of Borrelia burgdorferi. Two hundred sixty mice were captured from which there were 27 isolations. Significantly more mice were captured and significantly more isolations made from hemlock (Tsuga canadensis) habitat than from deciduous species forest. Hemlock habitat is sparse and focal but evidently increases winter survival of mice, and thus possibly results in increased infection rates in mice.