By T. Nguyen et al.
Isolated NK cells were labelled with fluorescent antibodies to determine TRPM3, CD107a, and CD69 receptors on CD56Dim CD16+ NK cells and CD56Bright CD16Dim/- NK cells. Ca2+flux and NK cytotoxicity activity was measured under various stimulants including PregS, Thapsigargin, 2APB and ionomycin. Unstimulated CD56Bright CD16Dim/- NK cells, significantly reduced TRPM3 receptors were seen in CFS/ME compared with HC. Ca2+flux showed no significant difference between groups.
Moreover PregS stimulated CD56Bright CD16Dim/- NK cells showed significant increase in Ca2+ flux in CFS/ME patients compared with HC. By comparison, unstimulated CD56Dim CD16+ NK cell showed no significant difference in both Ca2+ flux and TRPM3 expression. PregS stimulated CD56Dim CD16+ NK cells significantly increased TRPM3 expression in CFS/ME but this was not associated with a significant increase in Ca2+ flux. Furthermore, TG stimulated CD56Dim CD16+ NK cells increased K562 cell lysis prior to PregS stimulation in CFS/ME patients compared with healthy controls. Differential expression of TRPM3 and Ca2+ flux between NK cell sub-types may provide evidence for their role in the pathomechanism involving NK cell cytotoxicity activity in CFS/ME.
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Source: Nguyen T, Johnston S, Clarke L, Smith P, Staines D, Marshall-Gradisnik S. Calcium mobilisation in natural killer cells from Chronic fatigue syndrome/Myalgic encephalomyelitis patients is associated with transient receptor potential melastatin 3 ion channels.Clin Exp Immunol. 2016 Oct 11. doi: 10.1111/cei.12882. [Epub ahead of print]