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Detection of Borrelia burgdorferi by DNA amplification in synovial tissue samples from patients with Lyme arthritis.

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Abstract

OBJECTIVE:

To compare the detection rates of chromosomal flagellin gene from Borrelia burgdorferi in synovial tissue (ST) and synovial fluid (SF) using polymerase chain reaction (PCR) techniques.

METHODS:

B burgdorferi DNA was sought in SF and ST from 12 consecutive patients with
Lyme arthritis and from 29 patients with noninfectious diseases (controls).

RESULTS:

No DNA amplification was observed in samples obtained from the 29 control patients, whereas B burgdorferi DNA was detected in all ST and/or SF samples from the 12 patients with
Lyme arthritis. Results from 1 ST sample were not interpretable because of PCR inhibitors. Among the 11 remaining patients, 10 had positive ST samples, whereas only 4 had positive SF samples (P < 0.05).

CONCLUSION:

These data suggest that detection of chromosomal B burgdorferi DNA may be more efficient in ST than SF.

Arthritis Rheum. 1996 May;39(5):736-45. Comparative Study; Research Support, Non-U.S. Gov’t

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