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T2 Magnetic Resonance (T2MR®) Assay: A Useful Test for Detecting Borrelia

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Magnetic Resonance-based Direct Detection of Three Lyme Disease-Related Borrelia Species in Whole Blood Samples
 
Abstract
 
In early Lyme disease (LD), serological testing is insensitive and seroreactivity may reflect active or past infection. Here, we evaluated a novel assay for the direct detection of three species of Borrelia spirochetes in whole blood. The T2 Magnetic Resonance (T2MR®) assay platform was used to amplify Borrelia DNA released from intact spirochetes and to detect amplicon.
 
Analytical sensitivity was determined from blood spiked with known concentrations of spirochetes, and the assay's limit of detection was found to be in the single cell/mL range: 5 cells/mL for B. afzelii and 8 cells/mL for B. burgdorferi and B. garinii Clinical samples (N=66) from confirmed or suspected early LD patients were also analyzed.
 
B. burgdorferi was detected using T2MR in 2/2 (100%) of blood samples from patients with confirmed early LD, based on the presence of erythema migrans and documentation of seroconversion or a positive real-time PCR blood test. T2MR detected B. burgdorferi in blood samples from 17/54 (31%) of patients with probable LD, based on the presence of erythema migrans without documented seroconversion, or documented seroconversion in patients with a compatible clinical syndrome but without erythema migrans.
 
Out of 21 clinical samples tested by real-time PCR, only 1 was positive and 13 were negative with agreement with T2MR. An additional 7 samples that were negative by real-time PCR were positive with T2MR. Therefore, T2MR enables low LoD for Borrelia spp. in whole blood samples and is able to detect B. burgdorferi in clinical samples.
 
Source: By Snyder JL1, Giese H1, Bandoski-Gralinski C1, Townsend J1, Jacobson BE1, Shivers R1, Schotthoefer AM2, Fritsche TR2, Green C2, Callister SM3, Branda JA4, Lowery TJ1. T2 Magnetic Resonance-based Direct Detection of Three Lyme Disease-Related Borrelia Species in Whole Blood Samples.
J Clin Microbiol. 2017 May 31. pii: JCM.00510-17. doi: 10.1128/JCM.00510-17. [Epub ahead of print]
 
 
 

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